Protein_Domain
Fos
Part:BBa_M36501:Design
Designed by: Dominique Dabija, Christopher Jackson, Debha Amatya Group: Stanford BIOE44 - S11 (2011-12-03)
Fos-p1n
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 13
Illegal PstI site found at 55 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 13
Illegal PstI site found at 55 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 13
Illegal PstI site found at 55 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 13
Illegal PstI site found at 55 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design was modified to codon optimized for expression in E. coli bacterium.
Source
O'Shea, E., R. Rutkowski, W. Stafford, and P. Kim. "Preferential Heterodimer Formation by Isolated Leucine Zippers from Fos and Jun." Science 245.4918 (1989): 646-48. Print.